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Human Epigenome Consortium
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Data Analysis

  1. Genomic DNA undergoes a chemical treatment. The procedure converts all non-methylated cytosines into a different base, uracil (uracil's hybridization behaviour is identical to that of thymine), using the chemical bisulphite.
  2. The bisulphite treated DNA is amplified in a subsequent PCR reaction by using bisulphite specific primers.
  3. The PCR products are sequenced.
  4. The generated trace files undergo a data normalization that is specific for bisulphite converted sequences.
  5. Trace files can be visualized as shown in the example below or summarized in a matrix as shown in the data section.