- Genomic DNA undergoes a chemical treatment. The procedure converts all non-methylated
cytosines into a different base, uracil (uracil's hybridization behaviour is identical
to that of thymine), using the chemical bisulphite.
- The bisulphite treated DNA is amplified in a subsequent PCR reaction by using
bisulphite specific primers.
- The PCR products are sequenced.
- The generated trace files undergo a data normalization that is specific for
bisulphite converted sequences.
- Trace files can be visualized as shown in the example below or
summarized in a matrix as shown in the data section.